29th Annual Meeting of the American Society for Photobiology Downtown Marriott Chicago, IL . . . . . . . . . . . . . . . . . . . . . . July 7-12, 2001

SUNDAY, JULY 8

7:00-8:00am Chicago A-D		POSTER VIEWING I and Continental Breakfast
8:00-9:00am Chicago E		SCHOOL LECTURE I: How Do Plants "See" the Sun?, Paper 18 Pill-Soon Song Kumho Life and Environmental Science Laboratory, Kwangju, Korea and University of Nebraska, Lincoln, NE
9:00-12:00pm Chicago F-G		SYMPOSIUM 2: Photothermal Processes in Biology: Mechanisms and Applications, Papers 19-23 Chair: Michael Rodgers, Bowling Green State University, Bowling Green, OH Photothermal effects are capable of introducing localized modifications in biological systems. Such effects can be mediated by photosensitization involving either endogenous (melanin, hemoproteins) or exogenous (d-shell metallotetrapyrroles) chromophores that exhibit rapid and efficient radiationless decay from their primarily excited electronic states. The high fluence rates necessary can be provided with pulsed laser sources. This combination can lead to temperature spikes at least 150°C above the basal value in the immediate vicinity of the initial absorber. As a consequence, intense shock waves are generated which propagate through the surrounding cell/tissue compartments and induce extensive mechanical damage. The presentations included in this symposium will discuss the generation and dissipation of thermal energy in fluid media as a consequence of near IR light deposition in photosensitizing compounds. Examples of photothermal effects at both a cellular and in vivo level will also be presented. Attention will be focused on the mechanistic aspects of photothermal processes and definition of the factors that control the temporal and spatial scale of such photosensitization modality.
9:00am		The Generation of Thermal Energy Spikes using Ultrafast Laser Pulses and Photothermal Sensitizers, Paper 19 M. Rodgers Bowling Green State University, Bowling Green, OH
9:30am		Photosensitized Inactivation of Melanoma Cells by Photothermal Pathways: Mechanistic Studies and Phototherapeutic Applications, Paper 20 G. Jori University of Padova, Italy
10:00am		Selective Cell Targeting with Light-Absorbing Particles and Short Laser Pulses, Paper 21 C. Pitsillides1,2 and C. Lin1 1Massachusetts General Hospital/Harvard Medical School and 2Massachusetts Institute of Technology, Boston, MA
10:30am		Break
10:45am		In Vivo Photothermal Therapy with Palladium(II)-Octabutoxynaphthalocyanine, Paper 22 H. Diddens1,2, F. Fischer2, J. DeGroot2 and R. Pottier2 1Medizinisches Laserzentrum, Luebeck, Germany and 2Royal Military College of Canada, Kingston, Canada
11:15am		Controlled Photothermal Welding of Tissue: Experimental and Clinical Applications in Microsurgery and Ophthalmology, Paper 23 R. Pini1, L. Menabuoni2 and U. Reali3 1Istituto di Elettronica Quantistica, 2S.A. Oculistica, ASL 10 and 3Universita' di Firenze, Firenze, Italy
11:45am		Concluding Remarks G. Jori University of Padova, Italy
9:00-12:00pm Chicago E		SYMPOSIUM 3: Indoor Tanning: Optimizing Exposure Schedules and Sources, Papers 24-30 Chair: Sharon Miller, Food and Drug Administration, Rockville, MD and Howard Cyr, Food and Drug Administration, Rockville, MD This symposium supported in part by Wolff System Technology Corporation. Indoor tanning, both at commercial facilities and in the home, has become increasingly popular over the last few decades. Though 48-hour intervals between exposures are often recommended, some individuals choose to tan daily. Such exposure patterns can result in a very substantial UV burden which may contribute to skin cancer and photoaging, This symposium will review the possibilities of reducing UV exposure needed to produce a cosmetically-desirable tan by the use of optimal schedules and appropriately selected UV sources.
9:00am		Introduction S. Miller
9:10am		Recommended Exposure Schedules for Sunlamps and Tanning Beds: Past, Present and Future, Paper 24 W.H. Cyr U.S. Food and Drug Administration, Rockville, MD
9:25am		The Scientific Basis for Indoor Tanning Exposure Schedules, Paper 25 R. Sayre1,2 and M. Caswell1 1Rapid Precision Testing Laboratories, Cordova, TN and 2University of Tennessee Center for the Health Sciences, Memphis, TN
9:45am		Evolution of Sunlamp Spectra in the US Indoor Tanning Industry, Paper 26 M. Stepp Wolff System Technology Corporation, Marietta, GA
10:00am		Break
10:20am		Effect of Tanning Bed Exposure on Skin Pigmentation, Erythema, Blood Levels of Vitamin D and Gene Expression in the Skin, Paper 27 M. Holick, F. Chimeh, J. Flanagan and T. Chen Boston University Medical Center, Boston, MA
10:40am		Calculating Initial Session Exposure Times that Neither Overexpose, nor Underexpose Clients of Indoor Tanning Salons to Ultraviolet Radiation, Paper 28 D. Smith and P. Reykdal UVR Research Institute, Tucson, AZ
10:55am		Annual Exposure Limits: Why and How?, Paper 29 J. van der Leun University Medical Center, Utrecht, The Netherlands
11:15am		Evaluation of UV-Induced Tan vs. Time - Considerations for Development of Exposure Schedules, Paper 30 S. Miller, N. Lao, B. Zmudzka and J. Beer Food and Drug Administration, Rockville, MD
11:30am		Panel Discussion
9:00-10:45am Chicago H		PLATFORM SESSION 1:  Luminescence/Photosynthetic Pathways, Papers 31-36 Chairs: J. Woodland Hastings, Harvard University, Cambridge, MA and Paul Loach, Northwestern University, Evanston, IL
9:00am		Structural Requirements for Enzymatic Activity in a Single Catalytic Domain of a Dinoflagellate Luciferase, Paper 31 L. Liu and J.W. Hastings Harvard University, Cambridge, MA
9:15am		Bioluminescence of Mycetophilids (Diptera) from US and Australia, Paper 32 V. Viviani, J.W. Hastings and T. Wilson Harvard University, Cambridge, MA
9:30am		Real-Time Whole-Body Fluorescence Imaging of Bacterial Infection, Paper 33 M. Zhao, M. Yang, R. Hoffman AntiCancer, Inc. San Diego, CA
9:45am		Break
10:00am		Molecular Structure of the Luciferase Gene from the Dinoflagellate Pyrocystis lunula, Paper 34 O. Okamoto1, L. Liu1, D. Robertson2 and J.W. Hastings1 1Harvard University, Cambridge, MA and 2Clark University
10:15am		Interaction of the Core Light-Harvesting Complex with the Reaction Center in Photosynthetic Bacteria: Role of PufX, Paper 35 P. Parkes-Loach, C. Law, J. Chen and P. Loach Northwestern University, Evanston, IL
10:30am		Mapping Electronic Coupling within Photogenerated Radical Ion Pairs at Fixed Distances Using Magnetic Field Effects: Implications for Artificial Photosynthesis, Paper 36 M. Wasielewski, A. Lukas and P. Bushard Northwestern University, Evanston, IL
12:00-1:30pm		Lunch Break (on own)
1:30-2:30pm Chicago E		SPECIAL LECTURE I: ASP Research Award Lecture The Design and Synthesis of Artificial Photosynthetic Membranes, Paper 37 Thomas Moore Arizona State University, Tempe, AZ
2:30-5:30pm Chicago F-G		SYMPOSIUM 4: Quinones in Type 1 Reaction Centers, Papers 38-44 Chair: John Golbeck, Pennsylvania State University, University Park , PA Quinones in electron transfer systems are extremely versatile: they can function as the interface between electron transfer involving organic cofactors and electron transfer involving iron-sulfur clusters (as in Photosystem I), or between pure electron transfer and coupled electron/proton transfer involving a second organic cofactor (as in Photosystem II). Each quinone displays equilibrium binding and redox properties that can be very different for each site of interaction, and these properties are conferred largely by the protein environment. In this symposium, we will discuss two issues relevant to quinones in Photosystem I. One involves strategies for introducing alternative quinones into the A1 site, and the other focuses on the issue of whether electron transfer is unidirectional or bidirectional among the bifurcated electron transfer chain.
Chair's Remarks 2:30pm		Biological Incorporation of Quinones Into the A1 Site of Photosystem I, Paper 38 J. Golbeck1, B. Zybailov1, E. Shalome1 and G. Shen1 and P. Chitnis2 1Pennsylvania State University, University Park, PA and 2Iowa State University, Ames, IA
Speakers 2:55pm		Analysis of Mutations Near the Phylloquinones of Photosystem I, Paper 39 K. Redding1, B. Boudreaux1, F. MacMillan2, M. Guergova-Kuras3, R. Bittl4, K. Brettel5, P. Joliot3 and F. Gu1 1The University of Alabama, Tuscaloosa, Alabama, 2J.W. Goethe Universität, 3Institut de Biologie Physico-Chimique, 4Technicsche Universität, Berlin and 5CEA Saclay
3:20pm		Protein-Quinone Interactions in the Cyanobacterial Photosystem I, Paper 40 W. Xu1, W. Johnson1, B. Zybailov2, J. Golbeck2 and P. Chitnis1 1Iowa State University, Ames, IA and 2Pennsylvania State University, University Park, PA
3:45pm		Transient EPR Studies of Photosystem I in Quinone Biosynthetic Pathway Mutants, Paper 41 A. van der Est Brock University, St. Catharines, Canada
4:10pm		Photosystem I Reaction Centers with a Foreign Quinone in the A1 Site: Effect on Electron Transfer from AO- to Q, Paper 42 S. Savikhin, T.W. Johnson, P. Martinsson, P. Chitnis and W. Struve Iowa State University, Ames, IA
4:35pm		Characterization of Cyanobacterial Photosystem I Complexes Containing 2-Phytyl-1,4-Napthoquinone (Demethylphylloquinone), Paper 43 D. Bryant1, Y. Sakuragi1, B. Zybailov1, G. Shen1, P. Chitntis2, A. van der Est3, R. Bittl4, S. Zech5, D. Stehlik5 and J. Golbeck1 1Pennsylvania State University, University Park, PA, 2Iowa State University, Ames, IA, 3Brock University, St. Catherines, Canada, 4Technische Universität, Berlin, Germany and 5Freie Universität, Berlin, Germany
5:00pm		Photosystem I Electron Transfer Pathways as Studied by Multifrequency Time-Resolved Electron Paramagnetic Resonance Techniques, Paper 44 M. Thurnauer1 and G. Kothe2 1Argonne National Laboratory, Argonne, IL and 2University of Freiburg, Freiburg, Germany
2:30-5:30pm Chicago E		SYMPOSIUM 5: Photoimmunology: Multiple Mechanisms, Papers 45-48 Chair: Frances Noonan, George Washington University Medical Center, Washington, DC It is well established that UV radiation causes local and systemic alterations to the immune system in experimental animals and also in humans. These immune alterations appear to be important in skin cancer outgrowth and may impact on other sunlight related diseases. Several mechanisms have been postulated by which UV causes these effects. This symposium will attempt to present the evidence for these mechanisms with an emphasis on endeavouring to establish if there are common pathways involved.
2:30pm		Introduction F. Noonan
2:40pm		Perfusion of Cis-Urocanic Acid Over Peripheral Sensory Nerves Stimulates Neuropeptide Release, Paper 45 P. Hart1, Z. Khalil2, S. Townley1, M. Grimbaldeston1 and J. Finlay-Jones1 1Flinders University, Adelaide and 2University of Melbourne, Australia
3:15pm		Transcription-Coupled and Global Genome Repair Differentially Influence UVB-Induced Acute Skin Effects, Local and Systemic Immunosuppression, Paper 46 J. Garssen1, A. Boonstra2, A. Oudenaren2, H. Van Steeg1, B. Van Der Horst2, J. Hoeijmakers2 and H. Van Loveren1 1National Institute for Public Health and the Environment, Bilthoven, The Netherlands and 2Erasmus University, Rotterdam, The Netherlands
3:50pm		Break
4:10pm		UV Waveband Interactions Modulating Photoimmunosuppression, Paper 47 V. Reeve University of Sydney, Australia
4:45pm		Protection of the Delayed Type Hypersensitivity Immune Response to Candida Albicans and Alloantigen from Suppression by Chronic UV Radiation, Paper 48 F. Strickland1 and A. Darvill2 1The University of Texas M.D. Anderson Cancer Center, Houston, TX and 2The University of Georgia, Athens, GA
5:20pm		Panel Discussion
2:30-4:45pm Chicago H		PLATFORM SESSION 2:  Photodynamic Therapy I, Papers 49-56 Chairs: David Kessel, Wayne State University School of Medicine, Detroit, MI and Sandra Gollnick, Roswell Park Cancer Institute, Buffalo, NY
2:30pm		Bcl-2: The Target of 'Mitochondrial' Photodamage, Paper 49 D. Kessel and M. Castelli Wayne State University School of Medicine, Detroit, MI
2:45pm		Photodynamic Therapy Induces the Expression of Chemokines and Adhesion Molecules Crucial to Neutrophil Migration, Paper 50 S. Gollnick, S. Evans, B. Owczarczak, P. Maier, L. Vaughan, W. Wang and B. Henderson Roswell Park Cancer Institute, Buffalo, NY
3:00pm		Modulation of Fibroblast Activity and Collagen Synthesis and Raft Artificial Tissue Model Using Photodynamic Therapy, Paper 51 T. Yomo1, S. Ishimaru1, S. Wilson2, V. Wallace3 and B. Tromberg3 1Tokyo Medical University, Tokyo, Japan, 2University of California Irvine Medical Center, Irvine, CA and 3Beckman Laser Institute, Irvine, CA
3:15pm		Green Tea Antioxidant (-)-Epigallocatechin-3-Gallate Treatment to Normal Human Epidermal Keratinocytes Inhibits UVB-Induced Oxidative Stress-Mediated Phosphorylation of MAPK and EGFR Signaling Pathways, Paper 52 S. Katiyar1,2, F. Afaq1, A. Perez1, C. Elmets2 and H. Mukhtar1 1Case Western Reserve University, Cleveland, OH and 2University of Alabama, Birmingham, AL
3:30pm		Break
3:45pm		Identification and Characterization of Hypericin Analogs that Differ in Tumor Cell Toxicity, Paper 53 N. Wills, J. Park, J. Wen, S. Kesavan, G. Kraus, J. Petrich and S. Carpenter Iowa State University, Ames, IA
4:00pm		Modulation of Methylene Blue Photochemical Properties Based on the Adsorption at Aqueous Micelle Interfaces, Paper 54 H. Junqueira, M. Gugliotti, L. Dias and M. Baptista University of São Paulo, Brazil
4:15pm		PDT-Induced Death of Isolated Neuronal and Glial Cells, Paper 55 A. Uzdensky, A. Zhavoronkova, M. Kolosov and D. Bragin Rostov State University, Rostov-on-Don, Russia
4:30pm		Pre-Clinical Pharmacokinetic and Safety Assessment of Topical Rose Bengal, Paper 56 E. Wachter1, C. Dees1, J. Harkins1, T. Scott1, W. Fisher1, M. Petersen2 and P. Bjerring3 1Photogen, Inc. Knoxville, TN, 2The University of Tennessee College of Veterinary Medicine, Knoxville, TN and 3University of Aarhus, Aarhus, Denmark
5:30-7:30pm Chicago E		SPECIAL PRESENTATION: So You Want to be an NIH Grantee Paul Strudler Scientific Review Administrator, Radiation Study Section, National Institutes of Health, Bethesda, MD Many of you will drop your NIH application in the fedex box, and wave it bye-bye, much like a small child placing a boat in a fast moving creek. Six months later, if you don't have a good mentor, or if you haven't done this before (or even if you have done this before), you can get terrible news. I'm going to retrace your steps with you, repeating often used sayings from the peer review process to remind you of things not to do. I'm going to show you when and how to intervene and steer the process, what to do and not to do in writing a grant application, and for young investigators, I'm going to present a smorgasbord of applications for which you, and you alone, are eligible. Lastly, we'll throw in a few websites, some understanding of scoring grants, and remind you of the many uses of the SBIR program.
6:30pm Denver		EDITORIAL BOARD MEETING AND DINNER