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29th Annual Meeting of the American Society for Photobiology
Downtown Marriott
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WEDNESDAY, JULY 11
| 7:00-8:00am Chicago A-D |
POSTER VIEWING IV and Continental Breakfast | |
| 8:00-9:00am Chicago E |
SCHOOL LECTURE IV: Luminescence and
Fluorescence Reporters: Inspirations and Applications from Circadian Rhythms,
Paper 187 Carl Johnson Vanderbilt University, Nashville, TN |
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| 9:00-12:00pm Chicago F-G |
SYMPOSIUM 15: Singlet Oxygen: Chemistry to Biochemistry, Papers
188-193 Chair: Jeffrey Kanofsky, Hines Veterans Administration Hospital, Hines, IL This session will highlight recent work concerning the interactions of singlet oxygen with cells. In spite of the complexity of these biological systems, much progress has been made in our understanding of how singlet oxygen damages cells and how this damage might be prevented. |
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| 9:00am | Singlet Oxygen, Nitric Oxide, PhGPx
and Lipid Peroxidation, Paper 188 G. Buettner, F. Schafer, S. Venkataraman, E. Kelley and H. Wang The University of Iowa, Iowa city, IA |
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| 9:25am | Defense Mechanisms of Plant Pathogenic
Fungus Cercospora Against Singlet Oxygen Damage by the Phototoxin Cercosporin,
Paper 189 C. Chignell1, M. Daub2 and P. Bilski1 1National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC and 2North Carolina State University, Raleigh, NC |
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| 9:50am | Cholesterol as a Singlet Oxygen Detector
in Cell Membranes, Paper 190 A. Girotti1 and W. Korytowski2 1Medical College of Wisconsin, Milwaukee, WI and 2Jagiellonian University, Krakow, Poland |
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| 10:15am | Break | |
| 10:45am | Photosensitized Hemolysis of Red Blood
Cells - New Light on a Classical Photodynamic Action, Paper 191 L. Grossweiner Advocate Ravenswood Hospital Medical Center, Chicago, I |
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| 11:10am | Initiation of Apoptosis by Singlet
Oxygen, Paper 192 S. Zhuang, M. Lynch, C. Lin, I. Kochevar Harvard Medical School, Boston, MA |
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| 11:35am | Novel Photoprotective Properties of
Girard's-Reagent-P Derivative of ?-Apo-8'-Carotenal, Paper 193 J. Kanofsky1,2 and P. Sima1 1Hines VA Hospital, Hines, IL and 2Loyola University Stricht School of Medicine, Maywood, IL |
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| 9:00-12:00pm Chicago E |
SYMPOSIUM 16: ALA Photodynamic Therapy and Photodetection, Papers
194-196 Chair: Thomas Foster, University of Rochester, Rochester, NY This symposium sponsored in part by DUSA Pharmaceuticals. Since the pioneering work of Malik, Kennedy and Pottier more than a decade ago, research on themechanisms of ALA-based PDT and fluorescence photodetection has expanded greatly. The US FDA approval of ALA-PDT for actinic keratosis and the several promising clinical applications currently under investigation motivate the sustained interest in this fascinating and unique form of photosensitization. It is now appreciated that complex molecular, cellular and physiological factors may influence the synthesis and accumulation of ALA-induced protoporphyrin IX, and the understanding of the details of these dependencies will aid in the optimization of this approach to PDT and fluorescence detection of disease. The purpose of this symposium is to summarize some of our present knowledge of these issues and to indicate particularly promising near term research directions and clinical applications. |
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| 9:00am | Introduction T. Foster |
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| 9:10am | Short-Term Differentiation Therapy
and PDT, Paper 194 B. Ortel1, D. Sharlin1, D. O'Donnell1, E. Maytin2 and T. Hasan1 1Harvard Medical School, Boston, MA and 2Cleveland Clinic Foundation, Cleveland, OH |
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| 9:45am | Differential PPIX-Fluorescence and
Selective Tumor Toxicity Using 5-Aminolevulinic Acid: Metabolic Reasons
for a Clinically Relevant Phenomenon, paper 195 R. Knüchel1, R. Krieg1, J. Rauch1, H. Stepp2, D. Zaak2, R. Baumgartner2 1University of Regensburg, and 2University of Munich, Großhadern, Germany |
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| 10:20am | Break | |
| 10:40am | ALA Photodynamic Therapy: From Barrett's
to Brain, Paper 196 B. Wilson1,2, L. Lilge1,2, N. Marcon3 and P. Muller3 1Ontario Cancer Institute, University of Toronto, 2Photonics Research Ontario and 3St. Michael's Hospital, Toronto, Canada |
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| 11:15am | Current Status of DUSA Commercial Development of Levulan PDT Stuart Marcus DUSA Pharmaceuticals, Valhalla, NY |
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| 9:00-11:00am Chicago H |
PLATFORM SESSION 7: Phototechnology/Photobiology, Papers
197-203 Chairs: Reeder Sams, National Center for Toxicological Research, Jefferson, AR and Dennis Valenzeno, University of Kansas Medical Center, Kansas City, KS |
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| 9:00am | Simultaneous Detection of the FHIT
Gene and Protein Using Microarray-Based Biochip, Paper 197 M. Askari1,2, G. Miller1 and T. Vo-Dinh1,2 1Oak Ridge National Laboratory and 2University of Tennessee, Knoxville, TN |
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| 9:15am | A Fluorescent Temperature Sensor Based
on the Perylene/Exciplex Interconversion, Paper 198 N. Chandrasekharan, M. Lares and L. Kelly University of Maryland Baltimore County, Baltimore, MD |
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| 9:30am | Pressure Sensitive Polymeric Material,
Paper 199 A. Kimaro1, L. Kelly1, N. Chandrasekharan1, M. Hamner2 and M. Lares1 1University of Maryland Baltimore County, Baltimore, MD and 2Leading Edge Technologies, Columbia, MD |
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| 9:45am | Efficacy of the Sun Protection Film
Helioprotec, Paper 200 S. Grinschgl and P. Wolf University of Graz, Austria |
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| 10:00am | Break | |
| 10:15am | Improving Tanning Safety by Skin Typing
Using Skin Reflectance Measurements Combined with Questionnaires and Erythema
Effective Irradiation Measurement, Paper 201 E. Menzel1,3, D. Kockott2, R. Sippel3 1University of Applied Sciences, Dortmund, 2Kockott-UV-Technik, 3MSS Elektronik GmbH, |
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| 10:30am | Prevention of Short-Term Ultraviolet
Radiation-Mediated Damage by Resveratrol, Paper202 F. Afaq, H. Mukhtar and N. Ahmad Case Western Reserve University, Cleveland, OH |
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| 10:45am | Spectral-Based Determination of Edemal
Doses of Light from Multiple Sources Using Female SKH-1 Mice: Establishing
a Program for Conversion of Dosimeter Readings to Spectral-Based Irradiance,
Paper 203 R. Sams1,2, L. Couch1,2, B. Miller1,2, C. Okerberg3 and P. Howard1,2 1National Center for Toxicolgical Research, 2National Toxicology Program Center for Toxicology and 3Pathology Associates International, Jefferson, AR |
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| 12:00-1:30pm | Lunch Break (on own) | |
| 12:00-1:30pm Avenue Ballroom |
Mentoring Lunch (Kathryn Woodburn and David Kessel,
Co-Organizers) Sponsored by Pharmacyclics, Inc. |
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| 1:30-2:30pm Chicago E |
SPECIAL LECTURE V: Photodermatology:
From Phototherapy to Photoprotection, Paper 204 Henry Lim Henry Ford Hospital, Detroit, MI |
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| 2:30-5:30pm Chicago E |
SYMPOSIUM 17: Cardiovascular Photobiology, Papers 205-210 Chair: Kathryn Woodburn, Pharmacyclics, Sunnyvale, CA Photobiology has been therapeutically applied to an increasing number of cardiovascular indications. With conventional interventional tools there is a real risk of damage to the normal vessel wall while the hallmark of many photobiologic therapies is inherent selectivity. Nonclincial studies with many photosensitizers have demonstrated prevention of intimal hyperplasia and reduction in atheromatous plaque. Currently, two photosensitizers are undergoing clinical evaluation with many in preclinical testing. Here, preclinical, mechanism of action studies and the clinical viability of this new field will be discussed. |
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| 2:30pm | Opening Remarks K. Woodburn |
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| 2:40pm | Effect of Photodynamic Therapy on
Platelet Adhesion to Vascular Adhesive Proteins, Paper 205 P. Fungaloi1,2,3, R. Statius-van Eps2, Y-P. Wu1, J. Blankensteijn1, P. de Groot1, H. van Urk2 and G. LaMuraglia3 1University Hospital, Utrecht, The Netherlands, 2University Hospital, Rotterdam, The Netherlands and 3Harvard Medical School, Boston, MA |
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| 3:05pm | Intracoronary PhotoPoint Therapy for
Restenosis, Paper 206 J. Walker1, I. Leitch1, H. Kim2, H. Yazdi2, J. Roessler1, S. Rychnovsky1, R. Grove1 and R. Waksman2 1Miravant Medical Technologies, Santa Barbara, CA and 2Washington Hospital Center |
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| 3:30pm | Endovascular PDT with NPe6 Inhibits
Restenosis in Experimental Injured Arteries, Paper 207 T. Nagae1, K. Aizawa1, S. Ishimaru1, H. Nakai1, T. Saito2, M. Abe3 and K. Fujishima3 1Tokyo Medical University, 2Kyorin University, 3Meiji Seika Kaisha, Japan |
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| 3:55pm | Break | |
| 4:15pm | The PhotoPoint Catheter Based System
for the Treatment of Intimal Hyperplasia, Paper 208 R. Grove, S. Rychnovsky, M. Purter, R. Heath, I. Leitch and J. Walker Miravant Medical Technologies, Santa Barbara, CA |
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| 4:40pm | Antrin Photoangioplasty: Initial Human
Experience and Results of a Phase I Trial as Adjuvant Therapy to Intracoronary
Stent Implantataion, Paper 209 D. Kereiakes1, A. Szyniszewski1, D. Wahr2, H. Hermann3, D. Simon4, C. Rogers4, P. Kramer5, W. Shear6, A. Yeung7 , D.C. Adelman8 and T. Chou8 1The Linder Center, Cincinnati, OH, 2Michigan Heart and Vascular, Ann Arbor, MI, 3University of Pennsylvania, Philadelphia, PA, 4Brigham and Womens' Hospital, Boston, MA, 5Mid-America Heart Institute, Kansas City, MO, 6University of Minnesota, MN, 7Stanford University, Palo Alto, CA and 8Pharmacyclics, Sunnyvale, CA |
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| 5:05pm | Seeding of Photodynamic Therapy Treated
Vessels, Paper 210 P. Waterman1,2, M. Landis2, S. Tsai1, S. Kossodo1,2, G. Nigri1,2, P. Fungaloi1,2, M. Sherwood1 and G. LaMuraglia1,2 1Wellman Laboratories of Photomedicine and 2Division of Vascular Surgery, Massachusetts General Hospital, Boston, MA |
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| 2:30-5:30pm Chicago F-G |
SYMPOSIUM 18: Generation and Turnover of Peroxide Intermediates in
Photobiological Systems, Papers 211-215 Chair: Albert Girotti, Medical College of Wisconsin, Milwaukee, WI and Michael Davies, Heart Research Institute, Sydney, Australia This symposium sponsored in part by Bruker Instruments, Inc. Photodynamic action on biological targets such as nucleic acids, proteins, and lipids produces a plethora of reactive intermediates and products. The former include a variety of species broadly classified as peroxides, e.g. endoperoxides and hydroperoxides. If such species escape detoxification, they may undergo light-independent metal-catalyzed or thermal decomposition, thereby giving rise to free radicals which can amplify and extend the damaging effects of photoperoxidation alone. Little is known about such processes in the case of primary lipid hydroperoxides and much less in the case of protein and nucleic acid peroxides. This symposium will focus on recent developments in this area. |
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| 2:30pm | Opening Remarks A. Girotti and M. Davies |
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| 2:35pm | Photooxidation of Guanosine and Imidazole
Derivatives, Paper 211 C. Foote University of California, Los Angeles, CA |
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| 3:05pm | Quinone-Mediated Type I Photosensitized
Formation of Pyrimidine Hydroperoxides from 2'-Deoxyribonucleosides and
DNA, Paper 212 J. Cadet1, C. Bienvenu1, M. Berger1, J-L. Ravanat1 and R.J. Wagner2 1DRFMC/SCIB & UMR 5046, Grenoble, France and 2Université de Sherbrooke, Sherbrooke, Canada |
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| 3:35pm | Singlet Oxygen-Mediated Formation
of Protein Peroxides: Mechanisms and Biological Consequences, Paper
213 M. Davies1, A. Wright1, C. Hawkins1 and W. Bubb2 1The Heart Research Institute and 2University of Sydney, Australia |
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| 4:05pm | Break | |
| 4:15pm | Photoperoxidation of Unsaturated Lipids:
TYPE I vs TYPE II, Paper 214 L. R. Barclay Mount Allison Univeristy, Sackville, Canada |
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| 4:45pm | Photogeneration and Reductive Turnover
of Chloesterol-Derived Hydroperoxides, Paper 215 A. Girotti1, A. Vila1 and W. Korytowski2 1Medical College of Wisconsin, Milwaukee, WI and 2Jagiellonian University, Krakow, Poland |
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| 2:30-4:30pm Chicago H |
PLATFORM SESSION 8: Carcinogenesis/Melanin/Gene Therapy,
Papers 216-222 Chairs: Susan Fischer, University of Texas M.D. Anderson Cancer Center, Houston, TX and Dennis Oh, University of California, San Francisco, CA |
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| 2:30pm | A Mouse Model for UV-Induced Junctional
Melanoma, Paper 216 F. Noonan1, J. Recio2, H. Takayama2, P. Duray2, M. Anver3, E. DeFabo1 and G. Merlino2 1The George Washington University School of Medicine and Health Sciences, Washington, DC, 2National Cancer Institute, Bethesda, MD and 3Frederick Cancer Research and Development Center, Frederick, MD |
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| 2:45pm | Inactivating E2F1 Restores Apoptosis
Sensitivity and Cancer Resistance to Trp53-Deficient Mice, Paper
217 N. Wikonal1,2, E. Remenyik1,2, M. Liu1, D. Johnson3 and D. Brash1 1Yale University School of Medicine, New Haven, CT, 2University Medical School of Debrecen, Hungary and 3M.D. Anderson Cancer Center, Smithville, TX |
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| 3:00pm | Genetic Alterations in p16INK4a and
p14ARF Genes in Human Non Melanoma Skin Cancer, Paper 218 A. Pacifico1,3, A. Ouhtit1, L. Goldberg2, S. Bolshakov1, K. Peris3, S. Chimenti4 and H. Ananthaswamy1 1M.D. Anderson Cancer Center, Houston, TX, 2DermSurgery Associates, P.A., Houston, TX, 3University of l'Aquila, Italy and 4University of Tor Vergata, Rome, Italy |
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| 3:15pm | Constitutive Phosphorylation and Nuclear
Localization of Mutant p53 in UV-Induced Mouse Tumors, Paper 219
V. Melnikova, A. Santamaria, S. Bolshakov and H. Ananthaswamy University of Texas M.D. Anderson Cancer Center, Houston, TX |
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| 3:30pm | Break | |
| 3:45pm | Melanogenesis and BRCA1 Signaling
Pathways are Similar After UV Irradiation and Treatment with Bicyclic Monoterpene
Diols, Paper 220 M. Canning, J. Galvin, A. Brown, D. Brown and D. Yarosh AGI Dermatics, Freeport, NY |
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| 4:00pm | Searching for Relationships Between
the Structure and Function of Melanin, Paper 221 J.B. Nofsinger, C. Clancy and J. Simon Duke University, Durham, NC |
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| 4:15pm | Gene Profiling of UV-Induced Melanoma
of Monodelphis domestica, Paper 222 Z. Wang1, T. Dooley2, E. Robinson1 and J. McCarrey1 1Southwest Foundation for Biomedical Research, San Antonio, TX and 2IntegriDerm, L.L.C., Huntsville, AL |
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| 5:30-7:00pm Chicago A-D |
POSTER SESSION II, Papers 223-261 | |
| Bioluminescence, Paper 223
Cloning and Expression of the Active
Fragment of Luciferase from a Marine, Alga, Pyrocystis lunla, Paper
223 |
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| DNA Damage and Repair, Papers 224-228
HPLC-MS/MS Detection of UV-INduced
Pyrimidine Photoproducts Within Isolated and Cellular DNA, Paper
224 Thymine-Purine Base Vicinal Crosslinks:
Formation, Characterization and Measurement, Paper 225 Subcellular Localization of Pyrimidine
Dimer Glycosylases in Transfected HeLa-S3 Cells, Paper 226 Selective Guanine Oxidation Photosensitized
by the Antibacterial Fluoroquinolone Rufloxacin, Paper 227 Napthalimide Derivatives: Tunable
Nucleases and Proteases, Paper 228 |
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| Environmental Photobiology, Papers 229-230
Chlamydomonas nivalis: Light and
Photosynthesis in the Snow, Paper 229 Phytoplankton Photosynthesis in
Temperate Marine Ecosystems as Affected by Ultraviolet Radiation and Vertical
Mixing, Paper 230 |
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| Optical Dosimetry/Imaging/Spectroscopy, Paper 231-232
Variance-Optimized Monte Carlo Routine
for Fluorescence and Reflectance of Turbid Samples, Paper 231 Omnidirectional UV Fluence Measurements
using Iodide/Iodate Chemical Actinometry: Radiation Vessels and a Unique
Measuring Device, Paper 232 |
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| Oxidative Stress, Papers 233-236
Donors of NO and Pulsed Radiation
at 820nm Exert Effects on Cell Attachment to Extracellular Matrices,
Paper 233 Photosensitizer Generated Reactive
Oxygen Speeds Activation of the Slow Cardiac Delayed Rectifier Potassium
Current, Paper 234 Photocytoxicity and Photooxidative
Damage to Cellular Nucleic Acids Sensitized by Nanomolar Levels of Hypericin,
Paper 235 A Model for the Blue-Light Phototoxicity
of Ocular Lipofuscin, Paper 236 |
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| Photocarcinogenesis, Paper 237-238
Guidelines for Tanning Salon Owners,
Operators and Users, Paper 237 Photocarcinogenesis in the Tg.AC
Mouse: Lomefloxacin and 8-Methoxypsoralen, Paper 238 |
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| Photophysics, Paper 239
Light Driven Artificial Ion Pumps,
Paper 239 |
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| Photoreceptor Biology, Papers 240-241
Time-Resolved Optoacoustic Studies
of the Photochemically Active LOV2 Domain of Arabidopsis Phototropin,
Paper 240 Effect of Medium and Anion Substitution
on the Early Thermodynamics in Natronobacterium pharaonis Halorhodopsin
Photocycle, Paper 241 |
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| Psoralen Photochemistry/Photobiology, Papers 242-244
Role of Mitochondria in PUVA-Induced
Apoptosis, Paper 242 Preirradiated Psoralen Triggers
Apoptosis in a T-Cell Line, Paper 243 Differential Effect of 8-Methoxypsoralen,
4,6,4'-Trimethylangelicin and Chloropromazine on Cell Death and TNF-Alpha
Production, Paper 244 |
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| Sunscreens, Papers 245-248
Sunscreen Efficacy Measurements
Under Full Spectrum and UVB Filtered Solar Simulated Radiation Using the
Alkaline Comet Assay, Paper 245 The Importance of Using Broad Spectrum
High SPF Sunscreens, Paper 246 Two New Photo-Stable UVA/Broadband
Filters, Paper 247 In vitro Sunscreen Evaluations:
The Solution to Many Vexing Problems, Paper 248 |
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| UV Photobiology, Papers 249-255
Ovariectomy Accelerates Photoaging
of Rat Skin, Paper 249 Photochemical Oxygen Consumpsion,
Oxygen Evolution and Spectral Changes During UVA Irradiation of EMT6 Spheroids,Paper
250 Main Components of UV-Induced Tan:
Pigmentation and Erythema, Paper 251 DNA Damage Induced by Ultraviolet
Radiation vs. Melanin Content and Production in Human Skin Within Racial/Ethnic
Groups, Paper 252 Activation of the Human Elastin
Promoter by UVR is Decreased Through the Application of Antioxidants,
Paper 253 Acute and Sub-Chronic Effects of
Co-Treatment of Female SKH-1 Mice with Alpha- and Beta-Hydroxy Acids and/or
Simulated Solar Light, Paper 254 Ultraviolet A Radiation Favors Expression
and Substrate Supply for the Inducible Nitric Oxide Synthase in Normal
Human Skin, Paper 255 |
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| Vision, Papers 256-257
GeneChip Analysis of Human Lens
Epithelial Cells Exposed to UVA Radiation, Paper 256 Isobestic Regions Induced in the
Absorption Spectrum of Monomeric Bacteriorhodopsin by Changes in Light
Scattering, Paper 257 |
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| Digital Photobiology Compendium, Papers 258 - 261
History of Photobiology in the Digital
Photobiology Compendium, Paper 258 The Digital Photobiology Compendium
as a Learning Aid: From Undergraduate Through Professional, Paper
259 Basic Text Modules of the Digital
Photobiology Compendium, Paper 260 Advanced Monographs in the Digital
Photobiology Compendium, Paper 261 |
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| WORKS-IN-PROGRESS POSTERS (Titles Available at the ASP Registration
Desk) |
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| 7:30-11:00pm Illinois |
SECOND COUNCIL MEETING |