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Effect of Tanning
Bed Exposure on Skin Pigmentation, Erythema, Blood Levels of Vitamin
D and Gene Expression in the Skin
Holick, Michael1,
Chimeh, Farhad1, Flanagan, John 1
and Chen, Tai1
Boston University Medical Center1
Abstract-
We exposed 16 subjects ages 18 years and greater with skin types II-V
to a tanning bed 3 times per week for three months. Changes in skin
pigmentation, erythema-circulating concentrations of 25-hydroxyvitamin
D were measured at various intervals. A skin biopsy was obtained before
initiating the study and a skin biopsy was taken at the end of the study
from an exposed area and unexposed area. The buffy coat was collected
from the plasma. The skin and buffy coat were extracted for RNA and
real time quantitative RT-PCR was performed for several genes responsible
for proliferation, differentiation, circadian rhythm activity, vitamin
D metabolism and immunologic function. This work was supported in part
by USPHS MO1RR00533 and the Indoor Tanning Association.
Keywords: ultraviolet
radiation, skin, vitamin D, pigmentation
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