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Cytokine polymorphisms
play a role in the susceptibility to UVB-induced immunomodulation after
hepatitis B vaccination in human volunteers
Sleijffers, Annemarie1,
Yucesoy, Berran3, Garssen, Johan1,
Boland, Greet2, De Gruijl, Frank2,
Van Hattum, Jan2, Van Vloten, Willem2,
Luster, Michael3 and Van Loveren, Henk1
National Institute of Public Health and the Environment, Bilthoven,
The Netherlands1
University Medical Centre, Utrecht, The Netherlands2
National Institute for occupational safety and health, Morgan Town, West
Virginia, USA3
Abstract-
Over the last two decades it has become evident that UVB exposure (280-320
nm) impairs specific and non-specific immune responses, which has been
shown to play a significant role in photocarcinogenesis and in impairment
of resistance to certain skin as well as non-skin-associated infections.
More recently it is suggested that certain allergies, autoimmune diseases
and vaccination efficacy might also be affected by UVB exposure. In
the present study, the effect of erythemal artificial UVB (TL12 lamps)
on hepatitis B vaccination in human volunteers (191) was investigated
under controlled conditions. Volunteers were exposed to UVB on 5 consecutive
days (1 MED/day) followed by a standard hepatitis B vaccination protocol.
Although the UVB exposure regime was sufficient to suppress CHS responses
and NK activity, antigen-specific humoral (anti-HBs) and cellular immunity
(proliferation induced by HBsAg) were not significantly affected. For
all volunteers single nucleotide polymorphisms (SNPs) have been determined
for the following interleukines: IL-1RA (+2018), IL-1A (+4845), IL-1B
(+3953), TNF-A (-308) and TNF-A (-238). These polymorphisms, and most
importantly for IL-1RA, IL-1A and IL-1B, affect quantitatively the production
of the corresponding interleukines, and may thereby play a role in the
susceptibility to UVB-induced immunomodulation. Taking into account
these polymorphisms, it was demonstrated that humoral and cellular immune
responses to the hepatitis B vaccine as well as the susceptibility to
UVB-induced immunomodulation depends on the type of polymorphism. We
conclude therefore that UVB exposure prior to hepatitis B vaccination
does not affect either humoral nor cellular responses at a population
base. However, when study objects are subdivided according to their
interleukine polymorphism profile, differences in vaccination responses
as well as in UVB-induced immunomodulation of these responses can be
observed. This reveals an important issue regarding individual susceptibility
to UV that need to be taken into account when studying effects of UV
in humans.
Keywords: uv,
vaccination, polymorphisms, human
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